Fig. 6.
Role of Glut1 expression and inhibition in cell growth and apoptosis induction by perifosine, gemcitabine, and their combination. a Modulation of Glut1 mRNA levels in LPC028, LPC006, and PANC-1 cells after 24-h exposure to perifosine, gemcitabine, or to their combination, at IC50 values, as determined by qRT-PCR. b Representative of Glut1 membrane-bound expression in LPC006 and LPC028 cells exposed for 24 h to perifosine at IC50s; c Representative Western blot pictures of Glut1 expression in LPC006, LPC028, and PANC-1 cells exposed for 24 h to perifosine, gemcitabine, or their combination at IC50s. d Structure of the compound PGL13. e Cell growth inhibition in LPC006 cells after 72-h exposure to perifosine, gemcitabine, or to their combination, at IC50 values, together with DMSO or with the Glut1 inhibitor PGL13, at 30 μM. f Apoptosis induction by perifosine, gemcitabine, and their combination as assessed by bisbenzimide staining as described in the Methods section (insert: representative pictures of apoptotic LPC006 cells after treatment with perifosine and gemcitabine, original magnification ×40). Columns, mean values obtained from three independent experiments; bars, SEM. *Significantly different from cells treated with DMSO