Q-PCR analysis of ZnT and Zip transcript abundance in mouse small intestine. Each relative abundance was calculated by using 18S rRNA as the normalizer. Values given as means ± SEM (n = 3). Transcript levels in response to low (Zn-, <1 mg/kg) or high (Zn+, 150 mg/kg) Zn diet relative to adequate (ZnN, 30 mg/kg) Zn diet after the mice were fed for 21 days. (A) ZnT transcript levels. MT mRNA is shown as an example of a Zn-responsive gene. (B) Zip transcript levels. *, P < 0.05, compared with ZnN group.