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. 2017 Jan 7;11:1. doi: 10.1186/s13036-016-0043-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Extraction of the fluorescent model protein FbFP from E. coli BL21(DE3) pRhotHi-2 comparing the standard and HT protocol (according to Fig. 2a). a Fluorescence measurement of different fractions occurring during the extraction procedure applying the BioLector technique. Error bars indicate standard deviation of three simultaneously performed extraction experiments. b SDS-PAGE analysis of different fractions occurring during the extraction procedure. Target protein FbFP framed. Conditions: Cultivation in 10 mL OnEx auto-induction medium, 250 ml shake flask, n = 350 rpm, d₀ = 50 mm, 37 °C. Extraction with BugBuster 10× Protein Extraction Reagent