Fig. 3.

a Quadruples PCR profiles of 58 E. coli MDR isolates. Isolate IDs shown under each lane. Data assignment were performed according to the presence or absence of signals of the following gene order arpA, chuA, yjaA, TspE4.C2. Row 1, lanes 1, 12—group A (+−−−); lanes 2–11, 13, 15—groups A or C (+−+−), lanes 16–22—group B1 (+−+), lane 14—group B2 (−+++), lanes 23–24—group F (−+−); Row 2, lanes 1–2—group F (−+−), lanes 3–24—group B2 (−+++) or (−++−) in lane 7; Row 3, lanes 1–4—group B2 (−+++), lanes 5–8—groups E or D (++−+), lanes 9–10—groups A or C (+−+−). b Groups A/C differentiation. In all lanes, both bands for internal control and Group C specific trpA fragment (219 bp) are present. c Groups D/E differentiation. Lanes 1, 3 and 4—both bands for internal control and Group E specific arpA fragment (301 bp) are present. Lane 2—only internal control present