Table 2.

Multiplex PCR-specific primers for ESBL gene detection in Enterobacteriacaea

Primer name	β-Lactamase target	Sequence (5′–3′)	Length (bases)	Amplicon size (bp)	Reference
Multiplex I TEM, SHV	TEM variants including TEM-1 and TEM-2	Multi- F CATTTCCGTGTCGCCCTTATTC Multi- R CGTTCATCCATAGTTGCCTGAC	22	800	[10]
	SHV variants including SHV-1	Multi-F AGCCGCTTGAGCAAATTAAAC Multi-R ATCCCGCAGATAAATCACCAC	21	713
Multiplex II CTX-M group 1	Variants of CTX-M group 1 including CTX-M-1, CTX-M-3 and CTX-M-15	MultiCTX-M Gp1-F TTAGGAARTGTGCCGCTGYA MultiCTX-MGP1-R CGATATCGTTGGTGGTRCCAT	20	688
			21
Multiplex II CTX-M group 2	Variants of CTX-M group 2 including CTX-M-2	MultiCTX-MGp2-F CGTTAACGGCACGATGAC MultiCTXMGp2-R CGATATCGTTGGTGGTRCCA	18	404
			21
Multiplex II CTX-M group 9	Variants of CTX-M group 9 including CTX-M-9 and CTX-M-14	MultiCTX-MGp9-F TCAAGCCTGCCGATCTGGT MultiCTX-MGp9-R TGATTCTCGCCGCTGAAG	19	561
			18
Multiplex III CTX-M group 8/25	CTX-M-8, CTX-M-25, CTX-M-26 and CTX-M-39 to CTX-M-41	MultiCTX-MGg8/25-F AACRCRCAGACGCTCTAC MultiCTX-MGg8/25-R TCGAGCCGGAASGTGTYAT	18	326
			19