Table 3.

Primers used to amplify and sequence the seven housekeeping genes in the E. coli isolates for the MLST analysis

Gene	Primer sequences	Usage
adk	F 5′-ATTCTGCTTGGCGCTCCGGG -3′ R 5′-CCGTCAACTTTCGCGTATTT-3′	Amp/seq
fumC	F 5′-TCACAGGTCGCCAGCGCTTC-3′ R 5′-GTACGCAGCGAAAAAGATTC-3′	Amp/seq
gyrB	F 5′-TCGGCGACACGGATGACGG-3′ R 5′-ATCAGGCCTTCACGCGCATC-3′	Amp/seq
icd	F 5′-ATGGAAAGTAAAGTAGTTGTTCCGGCACA-3′ R 5′-GGACGCAGCAGGATCTGTT-3′	Amp/seq
mdh	F 5′-ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG-3′ R 5′-TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT-3′	Amp/seq
purA	F 5′-CGCGCTGATGAAAGAGATGA-3′ R 5′-CATACGGTAAGCCACGCAGA-3′	Amp/seq
recA	F 5′-CGCATTCGCTTTACCCTGACC-3′ R 5′-TCTCGATCAGCTTCTCTTTT-3′	Amp/seq