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. 2017 Jan 7;8:2. doi: 10.1186/s40104-016-0132-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effects of supplementing lactating sows with HMB on MyHC isoforms mRNA levels in skeletal muscle of the offspring at d 28 of age. Values are means, with their standard errors represented by vertical bars (n = 6). * Represents mean values between the two groups differ significantly at P < 0.05. Data was normalized against GAPDH, with results expressed relative to the CON sample using the ΔΔCt method (where Ct is cycle threshold) with efficiency correction. MyHC myosin heavy chain; CON control; HMB β-hydroxy-β-methylbutyrate