Table 3.
The primer sequences of the target genes
| Target gene | Primer sequence (5′to 3′) | Product size, bp | GenBank No. |
|---|---|---|---|
| MyHC1 | F: GTTTGCCAACTATGCTGGGG | 95 | NM_213855.1 |
| R: TGTGCAGAGCTGACACAGTC | |||
| MyHCIIa | F: CTCTGAGTTCAGCAGCCATGA | 127 | NM_214136.1 |
| R: GATGTCTTGGCATCAAAGGGC | |||
| MyHCIIx | F: TTGACTGGGCTGCCATCAAT | 111 | NM_001104951.1 |
| R: GCCTCAATGCGCTCCTTTTC | |||
| MyHCIIb | F: GAGGTACATCTAGTGCCCT | 83 | NM_001123141.1 |
| R: GCAGCCTCCCCAAAAATAGC | |||
| Pax7 | F: TGAAGGTCGGAGTGAACGGAT | 74 | NM_001206359.1 |
| R: CACTTTGCCAGAGTTAAAAGCA | |||
| IGF-1 | F: CACAGACGGGCATCGTGGAT | 90 | NM_214256.1 |
| R: ACTTGGCAGGCTTGAGGGGT | |||
| mTOR | F: CATTGGAGATGGTTTGGTGA | 160 | XM_003127584.5 |
| R: ATGGGATGTGGCTTGTTTGA | |||
| MRF4 | F: CCTTCGGTGCCTTTCTTCCAT | 88 | NM_001244672.1 |
| R: GAGTTATTTCTCCCCCACTTCC | |||
| MSTN | F: TTTACCTGTTTATGCTGATTGTTG | 194 | NM_214435.2 |
| R: TTTGCTAATGTTAGGAGCTGTTTC | |||
| FoxO1 | F: TCAAGGATAAGGGCGACAGC | 95 | NM_214014.2 |
| R: AATGTCATTATGGGGAGGAGAGT | |||
| Sox6 | F: CGGATTGGGGAGTATAAGCA | 159 | XM_01399454.1 |
| R: CATCTGAGGTGATGGTGTGG | |||
| MAFbx | F: CCCTCTCATTCTGTCACCTTG | 104 | NM_001044588 |
| R: ATGTGCTCTCCCACCATAGC | |||
| MuRF1 | F: GCTGGATTGGAAGAAGATGTAT | 144 | NW_001184756 |
| R: AGGAAAGAATGTGGCAGTGTCT | |||
| GAPDH | F: TGAAGGTCGGAGTGAACGGAT | 74 | NM_001206359.1 |
| R: CACTTTGCCAGAGTTAAAAGCA |
MyHC myosin heavy chain, Pax7 paired box 7, IGF-I insulin-like growth factor-I, mTOR mammalian target of rapamycin, MRF4 muscle regulator factor 4, MSTN myostatin, FoxO1 forkhead box transcription factor O1, MAFbx muscle atrophy F-box, MuRF1 muscle Ring finger 1, GAPDH glyceraldehyde-3-phosphate dehydrogenase