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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Semin Cell Dev Biol. 2016 Jul 7;61:140–149. doi: 10.1016/j.semcdb.2016.07.006

Figure 4.

Figure 4

Typical micrographs showing development of early preantral follicles obtained mechanically from the Peromyscus ovaries under in vitro culture in hanging drop of ovarian cell-conditioned medium: Five single follicles per drop on day 0 (A), one single aggregate formed from the five follicles on day 6 (B), high viability (green) of the follicle aggregate on day 6 (C), and the aggregate of antral follicles developed from the five early preantral follicles after continuous culture in hanging drop for 13 days (D); three follicles per drop on days 0 (E) and 6 (F) and the aggregate of antral follicles developed from the three early preantral follicles after continuous culture in hanging drop for 13 days (G); and only one follicle per hanging drop on day 0 (H) and the degenerated follicle on day 6 (I). The figure is reprinted and redrawn from reference [46] with permission from Mary Ann Liebert Inc.