Fig.4. The serine-substitution mutations reduce or abolish blue light-dependent degradation of CRY2.

(A–B) Immunoblot showing expression of the serine-substitution mutants of CRY2 in etiolated seedlings exposed to blue light. 6-day-old seedlings grown in dark were transferred to blue light (60 µmol m⁻² s⁻¹) for the indicated time (min). Proteins were extracted, fractioned by 10% SDS-PAGE gels, blotted, probed with anti-CRY2 (CRY2), stripped, and reprobed with anti-HSP90 antibody (HSP90) are shown.

(C–D) The relative level of CRY2 proteins calculated by normalization of the CRY2 signals in blue light-treated samples to that of the etiolated seedlings, and presented as CRY2^Blue (signal intensity of CRY2 in blue-light treated seedlings)/CRY2^Dark (signal intensity of CRY2 in seedlings before blue light treatment).