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Southern blot analysis of total nucleci acids of a healthy N. benthamiana (lane 1) and two separate N. benthamiana plants (lanes 2 and 3) agroinoculated with pBin20-1.4BCTIV-Kaf infectious construct hybridized with a 32P-labelled BCTIV probe. The positions of single-stranded (SS) DNA and open circular (OC), linear (lin), supercoiled (SC) dsDNA replicative-forms are indicated. Each lane was loaded with approximately 5 µg of total DNA
