Table 3.
Effect of metal ions and chemical reagents on CMCase activity
| Substances | Concentration (mM) | Residual activity (%) |
|---|---|---|
| Control | - | 100 ± 0.8 |
| ZnSO4 | 5 | 103 ± 1.1 |
| MgSO4 | 5 | 93 ± 0.8 |
| FeSO4 | 5 | 98 ± 1.2 |
| Hg2Cl2 | 5 | 30 ± 1.6 |
| CaCl2 | 5 | 150 ± 1.7 |
| CuSO4 | 5 | 160 ± 1.1 |
| MnSO4 | 5 | 118 ± 1.6 |
| KCl | 5 | 40 ± 0.9 |
| CoCl2 | 5 | 13 ± 0.8 |
| SDS | 5 | 60 ± 1.1 |
| H2O2 | 5 | 10 ± 0.9 |
| EDTA | 5 | 0.5 ± 0.02 |
| 2Me | 5 | 55 ± 0.7 |
| Tx100 | 5 | 105 ± 1.2 |
Activity was measured in the presence of 5 mM of each compound as mention in material and methods. Enzyme activity in no metal ions considered as 100%.