Figure 3. Comparison of V7 and C5 EL4 cell lines. Panel A: Whole-cell extracts from the indicated cell lines, equalized for protein, were immunoblotted for hemagllutinin (HA) to detect transfected HA-tagged phospholipase D2 (PLD2). V7 is the non-transfected parental cell line, C5 was transfected with catalytically active human HA-PLD2. D3 (not otherwise discussed herein) was transfected with catalytically-inactive HA-PLD2 (5). Panel B: V7 and C5 cells were incubated for 6 hours in a Boyden chamber in serum-containing medium. Cells that migrated through the insert were counted. Each data point represents the mean±SEM of triplicate wells.