Figure 2. RT-PCR analysis of C6 glioma cells with syndecan- and glypican-specific primers. Total RNA and cDNA was prepared from C6 glioma cells and subjected to RT-PCR analysis (refer to Materials and Methods and Table I for details). Lanes: M, 100 bp marker; 1, negative controls containing no cDNA; 2, C6 glioma cell line; 3; rat parathyroid cell line (positive control). SDC1-4: syndecan 1-4; GPC1-6: glypican 1-6.