Fig. 4.

Cl-amidine treatment has no effect on neutrophil migration to the peritoneum after CLP. Cells were collected from the peritoneum by injecting 5 ml of 1× PBS into the abdomen and then harvesting an equal volume. a The total number of cells as determined by a hemocytometer was not different between the Cl-amidine- and vehicle-treated groups. b, c Cytospins of peritoneal cells were Wright-stained and analyzed for neutrophil counts at ×20 magnification using an RGB, DIC N1 filter (0.33 µM/pixel). The number of neutrophils did not significantly differ between the vehicle control and the Cl-amidine-treated animals. d The percentage of Ly6G⁺ cells in the peritoneum in both groups at 24 h after CLP demonstrated no significant difference between the vehicle- and CL-amidine-treated animals, consistent with neutrophil counts. Sham, n = 4; CLP, n = 12 per group. * p < 0.05, one-way ANOVA.