Fig. 6.

Cl-amidine treatment significantly alters proinflammatory IL-6 cytokine levels in the spleen after CLP, but not in other tissues. Tissue homogenate lysates were normalized using a Bradford protein assay and were then assessed for IL-6 levels by ELISA. The tissues assessed were kidney (a), lung (b), spleen (c), and (d) liver 24 h after CLP. IL-6 levels were not significantly altered between the vehicle control and Cl-amidine-treated mice in all tissues except in the spleen, where Cl-amidine treatment significantly reduced IL-6 levels as compared to vehicle control CLP mice. Sham, n = 6; CLP, n = 12–16 per group. * p < 0.05 sham versus CLP, ^# p < 0.05 CLP vehicle versus CLP Cl-amidine, one-way ANOVA.