Fig. 4.

Nestin and GFAP expression in control and degenerated adult mouse retina.

Immunofluorescent labeling with nestin (green), GFAP (red), and DAPI (blue) is shown. Arrows indicate nestin/GFAP co-immunolabeling. The number of nestin+/GFAP+ Müller cells increased progressively at day 3 after MNU treatment, peaked at day 5, and decreased thereafter. GFAP-positive cells remained in an activated state at day 7 and day 21 post injection, despite the diminution in retinal thickness.

GFAP = glial fibrillary acidic protein, DAPI = 4-6-diamino-2-phenylindole, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer.