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. 2004 Oct 5;101(41):14895–14900. doi: 10.1073/pnas.0401168101

Fig. 1.

Fig. 1.

Localization of Egr-1, Cyr61, and CTGF in the lung tissues of GOLD-2 and GOLD-0 smokers. Egr-1, Cyr61 and CTGF expression, identified by SAGE and microarray analysis and confirmed by QRT-PCR, were examined for protein expression by immunofluoresence staining. (A-D) Egr-1 expression (red stain) was located in small airways (arrow) and alveolar epithelia (arrowhead) of GOLD-0 and GOLD-2 smokers. (E-H) Cyr61 expression (red stain) was located in small airways (arrow), alveolar epithelia (arrowhead), and inflammatory cells (star) of GOLD-0 and GOLD-2 smokers. (I-L) CTGF expression (green stain) was located in small airways (arrow), alveolar epithelia (arrowhead), small vessel endothelial cells (diamond), stromal cells (pentagon), and inflammatory cells (star) of GOLD-0 and GOLD-2 smokers. (Insets) Egr-1, Cyr61, or CTGF staining at higher magnification. Green (Egr-1 and Cyr61) and yellow (CTGF) stains are the autofluorescence. (Scale bars inside the box indicate 20 μm; scale bars outside the box indicate 100 μm.)