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. 2017 Jan 9;7:2035. doi: 10.3389/fpls.2016.02035

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Copyright © 2017 Annunziata, Ciarmiello, Woodrow, Maximova, Fuggi and Carillo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Asparagine synthetase (Asn1, Asn2, and Asn3) (A) and Δ1-pyrroline-5-carboxylate synthase (P5CS) (B) genes and their relative densitometric quantification normalized using the actin signals (C,D), the AS (E) and P5CS (F) enzymatic activities, the content of asparagine (G), and proline (H) in roots of control () and salt stressed () plants. Plants were subjected to salt stress starting from day 10 of culture. Control plants were grown without NaCl addition. Plants were harvested after 20 days of hydroponic culture. The values are mean ± SD (n = 4). Different letters above bars indicate significant difference between treatments (p < 0.05, LSD-test).

Inline graphic — Asparagine synthetase (Asn1, Asn2, and Asn3) (A) and Δ1-pyrroline-5-carboxylate synthase (P5CS) (B) genes and their relative densitometric quantification normalized using the actin signals (C,D), the AS (E) and P5CS (F) enzymatic activities, the content of asparagine (G), and proline (H) in roots of control () and salt stressed () plants. Plants were subjected to salt stress starting from day 10 of culture. Control plants were grown without NaCl addition. Plants were harvested after 20 days of hydroponic culture. The values are mean ± SD (n = 4). Different letters above bars indicate significant difference between treatments (p < 0.05, LSD-test).