Fig. 3.

Developmental expression of Sig-1R in OLs. (A-C) In vitro OLs at different stages of differentiation. Cells were triple-labeled with Sig-1R antibody, GalCer antibody, and filipin. (A) Early progenitor. (B) Immature OL. (C) Mature OL. (D) Sig-1R in corpus callosum of developing rat brains. Arrowheads show Sig-1R in interfascicular OLs. P, postnatal day. (E) Sig-1R (green) and A2B5-positive OL progenitors (red) in rat subventricular zone on postnatal day 1. LV, lateral ventricule. (F) Sig-1R (green) and MBP (red) in rat hippocampal CA2 on postnatal day 35. Arrowheads show myelin sheath identified as double outlines. ^*, A pyramidal neuron. (Bar, 20 μm.)