Fig. 3.

Subcellular localization of ubiquitinated Jun. YN-Ub and Jun-CC were expressed in COS-1 cells, and cells fixed 36 h after transfection were immunostained with antibodies directed against LAMP1 (A), LAMP2 (B), and Rab6 (C). (D) Xpress-tagged Itch was coexpressed, and the cells were immunostained with anti-Xpress antibody. Fluorescence images were collected by confocal microscopy by using filters selective for YN-CC (Left) and Alexa Fluor 594 (Center) fluorescence. The merged images are shown (Right).