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. 2017 Jan 9;7:659. doi: 10.3389/fphys.2016.00659

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Copyright © 2017 Tang, Blair, Walton, Málnási-Csizmadia, Campbell and Yengo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of pN-Bleb on in vitro motility. The sliding velocity in the in vitro motility assay was examined with M2β-S1 in the (A) absence (1% DMSO) and (B) presence of 50 μM pN-Bleb. The average sliding velocity was determined by examining 60 filaments from two different protein preps. The velocities were binned and fit to a Gaussian function to determine the mean ± SEM velocity in the presence and absence of pN-Bleb (195 ± 4.65 and 1334 ± 16.79 nm/s, respectively). (C) The average sliding velocities were plotted as a function of pN-Bleb concentration, which allowed determination of the IC50 for M2β-S1 (13.3 ± 0.1 μM) and Sk HMM (1.6 ± 0.2 μM).