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. 2017 Jan 9;7:659. doi: 10.3389/fphys.2016.00659

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Copyright © 2017 Tang, Blair, Walton, Málnási-Csizmadia, Campbell and Yengo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of pN-Bleb on ATPase Activity and ADP-release. (A) The actin-activated ATPase in the presence of 40 μM actin was determined as a function of pN-Bleb concentration, which allowed determination of the IC50 for M2β-S1 (12.3 ± 1.8 μM) and Sk HMM (0.43 ± 0.11 μM). The data at each pN-Bleb concentration represents the average ± SD from two separate preparations. (B) The ADP-release rate constant was determined in the presence and absence of pN-Bleb by mixing a complex of acto-M2β-S1.mantADP with excess ATP and monitoring the mant fluorescence decrease. The fluorescence transients displayed represent the average of 4–5 transients and are fit to a single exponential function.