Figure 6.

Impact of pN-Bleb on permeabilized myocardium muscle mechanics. Left-hand panels show raw data measured in pCa 4.5 solution plus 1.33% DMSO. Right-hand panels show data measured in pCa 4.5 solution plus 1.33% DMSO and either 1, 10, or 50 μM pN-Bleb. These values are normalized to the control (zero pN-Bleb) data measured for that preparation to improve statistical power. Post-hoc tests show the results of a linear mixed model statistical analysis as described in the main text. Each point shows data from a single preparation obtained from one of 4 hearts. (A) The maximum force was impacted by pN-Bleb in a dose-dependent manner. (B) The maximum power was reduced in the presence of pN-Bleb. (C) The maximum velocity and (D) rate of force development (k_tr) were unchanged by pN-Bleb.