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. 2017 Jan 9;7:674. doi: 10.3389/fimmu.2016.00674

Figure 5.

Figure 5

Enumeration of immune cells by surface staining at different time points post immunization. Splenocytes from Mw-HMN and HNM-alone immunized mice were used at for immunophenotyping at 24 h post-dose-1 (TP1), 3 weeks post-dose-1 (TP2), 24 h post-dose-2 (TP3), and 10 days post-dose-2 (TP4). (A) T helper cells, (B) T cytotoxic cells, (C) B cells, (D) macrophages, (E) NK cells, (F) granulocytes, and (G,H) DCs. Values represent mean % of gated splenocytes ± SD. Abbreviation: HNM, HA + NP + M2e. *p < 0.05, **p < 0.01, and ***p < 0.001.