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Enumeration of immune cells by surface staining post antigen stimulation. Splenocytes from Mw-HMN and HNM-alone immunized mice were stimulated with HNM and were used for immunophenotyping at 4 h post-stimulation (A–D) and 24 h post-stimulation (E–G). Abbreviations: US, unstimulated groups; S, stimulated groups; Mw, Mw-HNM groups; OP, only-protein groups (HNM); HNM, HA + NP + M2e. Values represent mean % of gated splenocytes ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001.
