Figure 7.

In vivo evaluation of zebrafish γδ T cells in B cell activation and IgM production. The degree of B cells activation is represented by the percentage of mIgM⁺CD40⁺ B cells measured by FCM and by the expression levels of IgM and CD40 detected by real-time PCR (A,B). In the flow cytometric analysis, different treatments are shown at the top of each block diagram. The data above the outlined area indicate the average percentages of mIgM⁺CD40⁺ B cells in each treatment group. In the real-time PCR assay, PCRs were run in combination with the endogenous β-actin control. Means ± SD of three independent experiments are shown. *P < 0.05, **P < 0.01. (C) The titers of IgM against KLH in each treatment group were examined by ELISA. The control groups immunized with KLH only. All of the groups were titered from 1/100 to 1/800, and the titer was ascertained based on the highest serum dilution at which the A450 ratio (A450 of postimmunization sera/A450 of preimmunization sera) is ≥ 2.1. The data of the background control group with PBS administration were subtracted from each experimental group.