Figure 2.

Levels of serine phosphorylation of p47^phox in diaphragm of patients with end-stage HF. (A) representative images of immunoblots, (B,D) levels of phosphorylated p47^phox normalized to total protein loaded into gel lane, (C,E) levels of phosphorylated p47^phox normalized to immunoblot signal of total p47^phox (from this figure). Controls (open boxes; n = 3) and HF patients (gray boxes; n = 9–11). Panels D and E are individual data from patients normalized to average of controls (dotted line)—y-axis labels as in (B,C), respectively. Representative protein gels are similar to that shown in Figure 1. ^*P < 0.05 by Mann-Whitney test.