FIGURE 2.

CCL-7 synergistically induces a significant increase in calcium influx in mouse BMMCs. Studies were performed with cultured BMMCs loaded with Fluo-4 AM. Fluorescence was measured over time before and after treatment with DNP-HSA with and without recombinant mouse CCL7. (A) Intracellular Ca²⁺ transient release and dose-response curves to CCL7. The arrow indicates the time of addition of DNP-has, with or without CCL7. The graph represents the average fluorescence intensity from cultured BMMCs. (B) The Fluo-4 ratio represents mean fluorescence after treatment (20–300 s)/mean baseline fluorescence before treatment (0–20 s). Data are mean ± SEM. The data shown are the average of the Fluo-4 ratio of four separate experiments. These studies were performed in the absence of extracellular calcium. *p < 0.05 from four experiments.