FIGURE 3.

CCL7 induces chemotaxis of RBL-CCR1 cells and mouse BMMCs. (A) After sensitization with anti-DNP IgE, 2 × 10⁴ RBL-CCR1 cells and BMMCs in serum-free medium were transferred to the upper compartment of a Transwell chamber; the lower compartment was filled with serum-free medium containing one of six concentrations (0–100 ng/ml) of recombinant human or mouse CCL7. Cells were incubated for 3 h before quantifying the number of migrated cells. (B) After sensitization with anti-DNP IgE, RBL-CCR1 cells were stimulated with 1 ng/ml recombinant human CCL7 or were left unstimulated. Then cells were fixed and processed for polymerized actin staining with TRITC-conjugated phalloidin. Original magnification ×40. (C) Ruffling for individual cells stimulated with control or CCL7 was assessed. For each group, 100 cells were counted per condition in three separate experiments. Data are mean ± SEM. *p < 0.05, **p < 0.01, ANOVA.