FIGURE 7.

CCL7 is upregulated during the early-phase responses of EAC. (A) Time course for development of EAC. Mice were immunized i.p. with OVA and alum every other week for a total of three times, rested for 7 d, and repeatedly challenged with eye-drop administration of OVA or saline every day for a total of six times. (B) Total RNA from samples of conjunctival tissue was isolated 30 min after final challenge, and Ccl7 expression levels were examined using real-time PCR. (C) Serum of mice was collected for ELISA analysis at 3 h after the final challenge. Data are mean ± SEM for n = 5 (B) and n = 10 (C) per group and are representative of four separate experiments. (D) Conjunctival tissues were fixed, serially sectioned, and stained for CCL7 using a polyclonal Ab. Localization of CCL7 (red) in the allergen-challenged conjunctiva of wild-type mice (b), saline (control)-challenged conjunctiva of wild-type mice (a), allergen-challenged conjunctiva of wild-type mice stained using the isotype-control Ab (c), and allergen-challenged conjunctiva of CCL7^−/− mice (d) was examined using immunofluorescence. Original magnification ×10. Blue, DAPI. *p < 0.05, **p < 0.01, ANOVA.