Figure 4.

Conserved in vitro expression of HDAC repressors and modulators of ARAC cytotoxicity. (a) 22 subunits of HDAC-1/-2 repressor complexes were detected by RNA-seq. (b) Scatterplots of RNA-seq counts from primary samples (x axes) versus cell lines (y axes) with points coloured to show components of CoREST (red), NuRD (purple) and SIN (green) or IDR value (blue: IDR<0.05; grey: IDR>0.05). Genes of interest with IDR>0.05 are labelled within plot. (c) Twenty-six genes associated with the 'cytidine pathway'³⁰ were detected including five membrane-bound transporters, 10 cytosolic processing enzymes and 11 genes whose expression levels correlate with ARAC sensitivity. (d) Scatterplots as for (b) with points coloured to show genes whose expression correlates with ARAC cytotoxicity (white), genes involved in ARAC processing (yellow) and transport (orange) or IDR value (blue/grey).