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. 2016 Dec 5;97(1):53–69. doi: 10.1038/labinvest.2016.127

Figure 4.

Figure 4

Silencing of the ASIC1a gene enhances the chemosensitivity of Bel7402/FU and HepG2/ADM cells. (a and b): ASIC1a protein expression levels were decreased in Bel7402/FU and HepG2/ADM cells by shRNA treatment. The silencing of ASIC1a in Bel7402/FU and HepG2/ADM cells was analyzed by western blotting. (c and d): Histogram showing the semiquantitative analyses of the gels from western blotting. Western blot bands are representative of three independent experiments. Data were expressed as the mean±s.d., ***P<0.001. (e and f): Chemosensitivity of Bel7402/FU-shASIC1a and HepG2/ADM-shASIC1a cells by MTT assay. MTT assays were conducted at 24 and 48 h. The concentrations of 5-FU were 25, 50, 100, 200, and 400 mg/l, and concentrations of ADM were 0.25, 0.5, 1, 2, and 4 mg/l. Data were expressed as the mean±s.d., *P<0.05 versus pH6.5+5FU or pH6.5+ADM group, **P<0.01 versus pH6.5+5FU or pH6.5+ADM group. (g and h): The effect of the activity of ASIC1a effect on drug resistance in Bel7402/FU-shASIC1a and HepG2/ADM-shASIC1a cells by MTT assay. MTT assays were conducted at 24 and 48 h. The concentration of 5-FU was 100 mg/l, the concentration of ADM was 1 mg/l, and the concentrations of amiloride were 25, 100, and 400 μmol/l. Data were expressed as the mean±s.d., *P<0.05 versus pH6.5+5FU or pH6.5+ADM group, **P<0.01 versus pH6.5+5FU or pH6.5+ADM group.