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. 2016 Dec 5;97(1):53–69. doi: 10.1038/labinvest.2016.127

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Copyright © 2017 The Author(s)

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Inhibition of the PI3K/AKT signaling pathway modulates the chemosensitivity of Bel7402/FU and HepG2/ADM cells. (a and b): Western blot analysis showed that the levels of PI3K and phosphorylated AKT were increased in Bel7402/FU and HepG2/ADM cells. (c and e, d and f): Histogram showing the semiquantitative analyses of the gels from western blotting. Western blot bands were representative of three independent experiments. The data are means±s.d. of three independent assays, **P<0.01 versus L-02 group, ***P<0.001 versus L-02 group. (g and i, h and j): The effect of the PI3K/AKT signaling pathway on the chemosensitivity of Bel7402/FU and HepG2/ADM cells by MTT assay. MTT assays were conducted at 24 and 48 h. The concentrations of wortmannin were 10, 20, 40, 80, and 160 μmol/l, the concentrations of MK-2206 2HCl were 7.5, 15, 30, 60, and 120 μmol/l, the concentration of 5-FU was 100 mg/l, and the concentration of ADM was 1 mg/l. The data are means±s.d. of three independent assays, *P<0.05 versus pH6.5+5FU or pH6.5+ADM group, **P<0.01 versus pH6.5+5FU or pH6.5+ADM group, ***P<0.001 versus pH6.5+5FU or pH6.5+ADM group.

Inhibition of the PI3K/AKT signaling pathway modulates the chemosensitivity of Bel7402/FU and HepG2/ADM cells. (a and b): Western blot analysis showed that the levels of PI3K and phosphorylated AKT were increased in Bel7402/FU and HepG2/ADM cells. (c and e, d and f): Histogram showing the semiquantitative analyses of the gels from western blotting. Western blot bands were representative of three independent experiments. The data are means±s.d. of three independent assays, **P<0.01 versus L-02 group, ***P<0.001 versus L-02 group. (g and i, h and j): The effect of the PI3K/AKT signaling pathway on the chemosensitivity of Bel7402/FU and HepG2/ADM cells by MTT assay. MTT assays were conducted at 24 and 48 h. The concentrations of wortmannin were 10, 20, 40, 80, and 160 μmol/l, the concentrations of MK-2206 2HCl were 7.5, 15, 30, 60, and 120 μmol/l, the concentration of 5-FU was 100 mg/l, and the concentration of ADM was 1 mg/l. The data are means±s.d. of three independent assays, *P<0.05 versus pH6.5+5FU or pH6.5+ADM group, **P<0.01 versus pH6.5+5FU or pH6.5+ADM group, ***P<0.001 versus pH6.5+5FU or pH6.5+ADM group.