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. 2016 Nov 22;116(1):66–76. doi: 10.1038/bjc.2016.379

Figure 4.

Figure 4

miR-137 increases the sensitivity of ovarian cancer cells to cisplatin-induced apoptosis. (A) SKOV3 cells were transfected with a mock, a mimic control, or a miR-137 mimic, and then the cells were treated with 10 μM cisplatin 24 h later. DAPI staining and TUNEL labelling of the cells' apoptotic nuclei were performed after 48 h. The apoptotic rate was expressed as the ratio of the number of the cells with TUNEL-positive nuclei to the total number of DAPI-positive cells. Representative experiments (scale bar, 100 μm) and the statistical results are shown in A. (B, C) A2780 (B) and primary EOC (C) cells were transfected with a mimic control and a miR-137 mimic as indicated, and the cells were treated with 10 μM cisplatin 24 h later. DAPI staining of the cells' apoptotic nuclei was performed after 24 and 48 h. The apoptotic rate was expressed as the ratio of the number of the cells with apoptotic nuclei to the total number of DAPI-positive cells. The average values±s.d. of three separate experiments were plotted, and significant differences from the control value are indicated by *P<0.05, ***P<0.001.