Table 1. Genotypes of mutants from unselected and selected libraries.
| Mutant | I268 | A271 | A272 | I310 | F313 | L436 |
|---|---|---|---|---|---|---|
| Unselected library | ||||||
| 1 | Deleted | Deleted | Deleted | Deleted | Deleted | Deleted |
| 2 | Deleted | Deleted | Deleted | Deleted | Deleted | Deleted |
| 3 | GTA(V) | CCT(P) | CCT(P) | TCG(S) | TCG(S) | Deleted |
| 4 | Deleted | Deleted | Deleted | Deleted | Deleted | Deleted |
| 5 | Deleted | Deleted | Deleted | Deleted | Deleted | GCG(A) |
| 6 | Deleted | Deleted | Deleted | Deleted | Deleted | Deleted |
| 7 | Deleted | Deleted | Deleted | Deleted | Deleted | Deleted |
| 8 | Deleted | Deleted | Deleted | Deleted | Deleted | Deleted |
| 9 | Deleted | Deleted | Deleted | Deleted | Deleted | TTC(F) |
| Selected library | ||||||
| 1 | GTG(V) | wtRXR | GTG(V) | TTG(L) | ATG(M) | TTG |
| 2 | GTG(V) | wtRXR | GCA | GTG(V) | TCC(S) | TTG |
| 3 | CTA(L) | GCT | GCA | ATG(M) | GTG(V) | TTG |
| 4 | GCG(A) | wtRXR | GCA | TCC(S) | GTG(V) | TTC(F) |
| 5 | GCT(A) | GCT | GCA | GCC(A) | GCG(A) | TTC(F) |
| 6 | GCT(A) | GCT | GTT(V) | GCC(A) | GCG(A) | TTC(F) |
| 7 | CTT(L) | GCT | GCT | GTC(V) | ATC(I) | TTG |
| 8 | CTG(L) | GTG(V) | GCG | TTG(L) | TTG(L) | TTG |
| 9 | GTG(V) | GTG(V) | GCG | TTG(L) | GTG(V) | TTG |
| 10 | GTA(V) | wtRXR | GTG(V) | ATG(M) | TCC(S) | ATG(M) |
| 11 | GCG(A) | GCG | GCA | ATG(M) | GCG(A) | ACG(T) |
| 12 | GCG(A) | GCT | GCG | TCG(S) | GTC(A) | TTC(F) |
Sequenced codons are followed by the encoded amino acid in parentheses. “wtRXR” indicates that the sequence corresponds to the wild-type RXR codon. “Deleted” indicates the presence of an unmutated 3Stop deletion background cassette.