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. 2004 Sep 30;101(41):14901–14906. doi: 10.1073/pnas.0406196101

Fig. 1.

Fig. 1.

ALF family protein structure. (A) The conserved N- and C-terminal homology (NHD/CHD) and ALF domains are shown and the yeast two-hybrid bait region is marked. (B) Multiple amino acid alignment of the ALF family including the 23-mer Af4 peptides used for the Siah-1a dissociation-enhanced lanthanide fluorescence immunoassay. GenBank accession nos. from top to bottom: NP_598680, AY749168, NP_005926, NP_032058, NP_002016, XP_283603, NP_002276, NP_291043, and NP_055238; Fugu rubripes and Danio rerio sequences were taken from ENSEMBL database-predicted proteins SINFRUP00000169375 and ENSDARP0000009290, respectively, which can be accessed at www.ensembl.org. The Siah-binding sequence of PHYL (NP_725394) is shown, in addition to the consensus motif (18).