Fig. 3.

Trehalose increases LC3-II and autophagic flux. (A) Immunocytochemistry of cells exposed for 14 days to 2.5 µg/mL PFFs and probed for LC3 (green) and counterstained with nuclear dye Hoechst (blue). Images were taken at 63×. (B) Quantification of images from A. (C) Autophagic flux analysis of neurons exposed for up to 14 days to PFF with and without trehalose and then to 40 µM chloroquine (CQ) 5 h before protein collection. Control (Con) was 14 days without Trehalose. (D) Quantification of western blot C. Data represent mean±SEM (n=3). *p<0.05 compared to control; the Student t-test. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)