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. 2004 Oct 1;101(41):14806–14811. doi: 10.1073/pnas.0403959101

Fig. 2.

Fig. 2.

GAF and Mod(mdg4) interact directly in vitro and in a two-hybrid assay. The relevant protein domains of the proteins are indicated. (A) Interaction between His-tagged GAF and in vitro-translated Mod(mdg4)-67.2 and the luciferase negative control. (B) GST pull-down by using GST-fused Mod(mdg4)-67.2 and the Mod(mdg4) BTB domain or GST alone as the negative control. The various GAF derivatives were produced by in vitro translation. (C) Yeast two-hybrid assay. –, +, ++, and +++ represent no, weak, middle, and strong interaction, respectively.