Figure 1.

A) Representative immunoblots of heart myeloid differentiation factor 88 (MyD88) protein content (upper traces above the columns) in mice following 4 and 8 hr treatment with LPS (both 1 and 2 mg/kg, IP). Bars represent the ratio of MyD88 to GAPDH. ^***P<0.001 vs. control 8 hr, using one way ANOVA with LSD post hoc test (L: LPS and C: control). B) Expression of Tlr-4 mRNA in mice myocardial tissues following 4 and 8 hr treatment, with LPS (2 mg/kg, IP). The expression of Tlr-4 mRNA was analyzed by real time PCR. Data are expressed as the mean±SEM of the three independent experiments with three repeat. The significant of results was tested by a Pair Wise Fixed Reallocation Randomization Test using REST software. Values are mean±SEM (n=4). ^***P <0.001 from respective control value