Fig. 3.

The inhibitory activity is independent of auxin structure and reversible for most compounds. (A) Five-day-old BA3 seedlings were incubated with the indicated concentration of inhibitor and auxin for 4 h, followed by staining with X-gluc. 2,4-D, 2,4-dichlorophenoxyacetic acid. (B) Five-day-old BA3 seedlings were incubated with the indicated concentration of inhibitor (24-h wash) or inhibitor/IAA (prewash) for 4 h. The seedlings were then stained with X-gluc (prewash) or incubated in fresh medium before an additional 4-h treatment with IAA and subsequent staining with X-gluc (24-h wash).