Figure 3.

Synergistic functions of p53 overexpression and DNC treatment in inducing apoptosis in U87-MG cells. (A-D) The results of flowcytometry: Viable cells (Annexin-V-/PI-), early apoptotic cells (Annexin-V+/PI-), late apoptotic cells (Annexin-V-/PI+), and necrotic cells (Annexin-V+/PI+) are located in the lower left, lower right, upper right, and upper left quadrants, respectively. (E) Graphic representation of the percentage of apoptotic cells for control, p53 overexpressed, DNC treated and p53 overexpressed + DNC treated samples. When cells are both treated with DNC and transfected with p53, the number of cells undergoing apoptosis is significantly increased compared with the condition when cells are exposed to DNC treatment or p53 overexpression alone. PI: Propidium iodide