Figure 5. Mechanism study for the roles of G6PI plays during the regulation of hypoxia-induced angiogenesis.

(A) Supernatant of RASFs was collected and assayed for VEGF abundance by ELISA demonstrating the induced VEGF secretion from RASFs by Hypoxia (left). In addition, hypoxia increased the mRNA expression of VEGF, β-FGF, Ang1 and Ang2 in RASFs (right). (B) Decreased expression of VEGF in RASFs by treatment with G6PI-siRNA at the protein level (left and middle). Decreased VEGF secretion from RASFs by treatment with G6PI-siRNA (right). (C) HIF-1α-siRNA inhibited the expression of G6PI in RASFs. (D) G6PI-siRNA did not affect the expression of HIF-1α in RASFs. Data were presented as mean ± SEM(n = 3). *P < 0.05.