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. 2017 Jan 9;7:40018. doi: 10.1038/srep40018

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Copyright © 2017, The Author(s)

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(A) The MoATG14 locus and gene deletion vector. Arrows 1–8 indicate the primers ATG14up-1/2, ATG14dn-1/2, HPH-1/2 and ATG14-N1/2. (B) Southern blot analysis of ΔMoatg14 mutants 1 and 2, and the wild-type strain Guy11. Genomic DNA was digested with NcoI and separated on a 0.7% agarose gel. The DNA was hybridized with the probe (indicated in A) amplified from genomic DNA of Guy11.