Fig. 2.

Fusogenic activities of TMS peptides. (A) Typical fusion kinetics reveal that fusogenicity depends on the Leu/Val ratio of the hydrophobic peptide core sequences. P/L ratios were from 0.0035 to 0.005, except for peptide V16, which was incorporated up to a P/L ratio of only 0.0011. (B) Quantitative comparison of fusogenicities of the different aliphatic core sequences by plotting fusion extents, as seen after 1 h, or initial rates (Insets) as a function of P/L ratio. (C) Fusogenicities of peptides containing Gly and/or Pro relative to the parental LV16. Data points represent means ± SE (n = 4–18 independent experiments).