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Expression of the constitutively active Arf6 mutant Arf6/Q67L inhibits virus particle production. HeLa cells were transfected with pNL4-3 alone (-) or were cotransfected with pNL4-3 and an expression plasmid encoding HA-tagged Arf6/Q67L (+) and were analyzed as in Fig. 1. Virus release efficiency was calculated based on data from four independent experiments as described in Materials and Methods. Data are shown as means ± SD.
