Table 3. Exclusivity (specificity) analysis.
| Organism Typea | Organism name | Signal (Y/N)b |
|---|---|---|
| Virus | Human norovirus GII.4 Sydney strain | Y (4/4) |
| Virus | Human norovirus GI.6 | N |
| Virus | Human norovirus GII.3 | Y (1/3) |
| Virus | Poliovirus 1 | N |
| Virus | Feline calicivirus | N |
| Virus | Tulane virus | N |
| Virus | Adenovirus 41 strain Tak (ATCC VR-930D) | N |
| Virus | Hepatitis A virus | N |
| Virus | Bacteriophage MS2 (ATCC 15597-B1) | N |
| Enteric bacteria | Escherichia coli (Migula) Castellani and Chalmers Strain C3000 (ATCC 15597) | N |
| Enteric bacteria | Escherichia coli O157:H7 | N |
| Enteric bacteria | Enterobacter cloacae subsp. Cloacae (Jordan) Hormaeche and Edwards, subsp. nov. (ATCC 13047) | N |
The genomic RNA/DNA of several enteric viruses and bacteria was extracted using a NucliSens EasyMAG (bioMerieux), and 10−2 and 10−3 dilutions of the extracts were loaded as template in the RT-RPA assay using the G2F5-G2R11-G2P1 primer-probe set.
cShowed low reactivity in one replicate.
aSource organism type used. All organisms may be present in human enteric samples.
bWhether or not an amplifiable signal was observed at any point for any dilution with RT-RPA. If yes, then the proportion of replicates for which a positive signal was obtained is presented in parentheses.