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. 2017 Jan 9;7:39863. doi: 10.1038/srep39863

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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SzV-1287 at 10 μM and LJP-1207 at 10 μM were added to the culture medium and were present continuously started from day 6 or day 8. (A) Representative picture of the metachromatic cartilage areas in 10-day-old micromass cultures were visualised with DMMB dissolved in 3% acetic acid. Metachromatic (purple) areas represent cartilaginous nodules formed by many chondrocytes embedded into a cartilage matrix rich in polyanionic glycosaminoglycan (GAG) chains. Original magnification was 4x. Scale bar, 500 μm. (B) Optical density (OD625) was determined in samples containing TB extracted from micromass cultures with 8% HCl dissolved in absolute ethanol. Asterisks indicate significant (*p < 0.05) alterations of the amount of extracted TB (reflecting on the amount of GAG-rich matrix) as compared to the respective control. Representative data of 3 independent experiments exhibiting the same patterns of changes are shown.