Table 1. The discovery experiment is confirmed by SRM.
| Peptide Sequence | Protein Descriptions | SRM | Disc. Exp. | |||
|---|---|---|---|---|---|---|
| FC | P | CV (%) | FC | P | ||
| aLTVPELTQQMFDAk | Tubulin beta-3 chain | 1.60 | 5.3e-6 | 13.8 | 1.56 | 3.9e-3 |
| eQFYHk | Hydroxymethylglutaryl-CoA synthase, mitochondrial | 1.01 | 4.6e-1 | 13.8 | 1.40 | 6.8e-3 |
| eTLMDLGTk | Carbamoyl-phosphate synthase [ammonia], mitochondrial | 1.1 | 1.5e-3 | 7.5 | 1.40 | 1.1e-2 |
| iLTFDQLALESPk | 60S ribosomal protein L18 | 1.62 | 5.3e-5 | 13.1 | 1.47 | 2.2e-5 |
| rFEYEDPFFNR | Cytochrome P450 2D26 | 1.14 | 8.4e-2 | 10.0 | 1.47 | 4.3e-5 |
Detected fold changes (FC) and p-values (P) of 5 of the 6 successfully analysed peptides through the SRM method are presented and compared to fold changes and p-values from the discovery experiment. The average technical repeat CV Value (%) (CV) from the SRM validation is indicated.