Figure 2. OA attenuated the high glucose-induced impairment of Akt-Ser⁴⁷³ and eNOS-Ser¹¹⁷⁷ phosphorylation.

(a) HUVECs were pretreated with or without OA (10 μM) for 12 h, and then exposed to normal (5 mM) or high (30 mM) glucose for 12 h. Protein levels of p-eNOS, eNOS, p-Akt and Akt were detected by using western blotting. (b) Quantification of p-eNOS/eNOS and p-Akt/Akt. (c) HUVECs were stimulated with OA (10 μM) for 24 h, cell lysates were analyzed to determine p-eNOS, eNOS, p-Akt and Akt protein levels by using western blot. (d) Quantification of p-eNOS/eNOS and p-Akt/Akt levels in HUVECs. Data were shown as mean ± SEM of independent experiments. *P < 0.05 vs. vehicle control. ^#P < 0.05 vs. HG.